Volume 48 (2002) No. 6
Relevant Animal Model of Human Lymphoblastic Leukaemia/Lymphoma – Spontaneous T-Cell Lymphomas in an Inbred Sprague-Dawley Rat Strain (SD/Cub)
B. OTOVÁ1, M. SLADKÁ1, J. DAMOISEAUX2, A. PANCZAK1, V. MANDYS3, I. MARINOV4..............................213
1Institute of Biology and Medical Genetics, 1st Faculty of Medicine and General Teaching Hospital, Charles University, Prague, Czech Republic
2Department of Experimental and Clinical Immunology, University Maastricht, Maastricht,
The Netherlands
3Department of Pathology, 3rd Faculty of Medicine, Charles University, Prague, and Department of Teratology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
4Institute of Haematology and Blood Transfusion, Clinical Department, Prague, Czech Republic
Corresponding author: Berta Otová, Institute of Biology and Medical Genetics, 1st Faculty of Medicine and General Teaching Hospital, Charles University, Prague, Czech Republic, Albertov 4, 128 00 Prague, Czech Republic. E-mail: boto(zavináč)lf1.cuni.cz.
Abstract.
Full text. 213-218 219-226
Original Articles
Analysis of Transcription of Plant 7SL RNA Gene Variants in HeLa In Vitro Transcription System
L. VRBA1, 2, J. MATOUŠEK1.................................227
1Department of Molecular Genetics, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
2University of South Bohemia, Faculty of Biological Sciences, České Budějovice, Czech Republic
Corresponding author: Jaroslav Matoušek, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic. Tel.: +420 38 777 5529; Fax: +420 38 53 00 356; e-mail: jmat@umbr.cas.cz.
Abstract.
Full text. 227-231
Role of a Capacitation-Related Protein on Some Sperm Functional ParametersM. MOLLOVA1, R. NEDKOVA1, M. IVANOVA1, Tz. DJARKOVA1, J. PEKNICOVA2, S. KYURKCHIEV1..............................232
1Institute of Biology and Immunology of Reproduction, Sofia, Bulgaria
2Institute of Molecular Genetics, Prague, Czech Republic
Corresponding author: Margarita Vassileva Mollova, Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, 73, Tzarigradsko shosse, Blvd; Sofia 1113, Bulgaria.
Abstract.
Full text. 232-236
Rosiglitazone Improves Insulin Resistance, Lipid Profile and Promotes Adiposity in a Genetic Model of Metabolic Syndrome X
O. ŠEDA1,2, L. KAZDOVÁ2, D. KŘENOVÁ1, V. KŘEN1,3....................237
1Institute of Biology and Medical Genetics, 1st Medical Faculty, Charles University, Prague, Czech Republic
2Institute for Clinical and Experimental Medicine, Prague, Czech Republic
3Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Corresponding author: Vladimír Křen, Institute of Biology and Medical Genetics, 1st Medical Faculty, Charles University, Prague, Albertov 4, 12800 Prague 2, Czech Republic. Tel.: +420 224 968 147; email: vkren(zavináč)lf1.cuni.cz.
Abstract.
Full text. 237-241
Short Communication
Freezing and Thawing of Murine Bone Marrow-Derived Dendritic Cells Does Not Alter Their Immunophenotype and Antigen Presentation Characteristics
L. MENDOZA1, J. BUBENÍK1, M. INDROVÁ1, J. BIEBLOVÁ1, V. VONKA2, J. ŠÍMOVÁ1...........................................................................242
1Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
2Institute of Haematology and Blood Transfusion, Prague, Czech Republic
Corresponding author: Luis Mendoza, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 37 Praha 6, Czech Republic. Tel.: +420 220 183 461; Fax: +420 224 310 955.
Abstract.
Full text. 242-245
DicodonUse: The Programme for Dicodon Bias Visualization in Prokaryotes
J. PAČES, V. PAČES 1,2.........................246
1Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
2Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic
Corresponding author: Jan Pačes, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo 2, 16637 Prague 6, Czech Republic. Fax: +420 (2) 24311019; e-mail: paces@img.cas.cz.
Abstract.
Full text. 246-248
Relevant Animal Model of Human Lymphoblastic Leukaemia/Lymphoma – Spontaneous T-Cell Lymphomas in an Inbred Sprague-Dawley Rat Strain (SD/Cub)
B. OTOVÁ, M. SLADKÁ, J. DAMOISEAUX, A. PANCZAK, V. MANDYS, I. MARINOV
More than a decade of experimental work in an inbred subline of Sprague-Dawley rats having high incidence of spontaneous T-cell lymphoma/leukaemia is reviewed. Longitudinal follow-up of biological characteristics (growth, survival, haematology) of both multiple cases of primary disease and s.c. passaged lymphomas as well as comparative immunophenotypic and karyotypic studies are concluded. In these T-cell lymphomas (mostly CD4 positive), arising on the same genetic background of the inbred SD strain, the aberrations involving chromosome 11 have been recognized as a typical non-random cytogenetic marker. This unique rat model of lymphoblastic lymphomas/leukaemias, relevant to human pathology, seems to be very suitable for testing different anticancer therapeutic strategies, as it is documented by results of a number of various protocols conducted in our laboratory.
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Original Articles
Analysis of Transcription of Plant 7SL RNA Gene Variants in HeLa In Vitro Transcription System
L. VRBA, J. MATOUŠEK
We have performed an analysis of transcription of hop 7SL RNA genes in heterologous human extract from HeLa cells. Several variants of the Hl7SL-1 gene with a truncated or mutated 5’ non-transcribed part revealed a crucial importance of TATA box for transcription. The USE element was found important but dispensable for transcription. Transcription of mutants in the A-like box and experiments with hop 7SL RNA pseudogenes E44 and G32 revealed the importance of internal elements. The A-like box and possibly CG doublet at position +15/+16 described by Bredow et al. (1990a) are according to our results indispensable for in vitro transcription of plant 7SL RNA genes in human extract.
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Role of a Capacitation-Related Protein on Some Sperm Functional ParametersM. MOLLOVA, R. NEDKOVA, M. IVANOVA, Tz. DJARKOVA, J. PEKNICOVA, S. KYURKCHIEV
Abstract. In previous studies a series of Mabs against boar capacitated sperm have been produced. One of these Mabs – 4B12 – was found to recognize a surface membrane-associated protein located in the acrosome portion of the spermatozoa that became accessible to antibody after capacitation. In biological experiments it was shown that Mab 4B12 significantly inhibited boar sperm-porcine ZP binding. In attempts to investigate the mechanisms by which Mab 4B12 affected sperm-ZP binding, the role of the cognate protein on some functional parameters such as sperm motility and ability of the capacitated spermatozoa to undergo AR was studied. Experimental models of premature AR and AR physiologically induced with ZP were applied to study the effect of Mab 4B12 on boar sperm AR using PSA staining to estimate the acrosome-reacted state of spermatozoa. Sperm motility characteristics were determined by the time-exposure photokinesigraphic method. The results obtained in the present study, together with previously established inhibition of sperm-ZP binding by Mab 4B12, documented the participation of the 4B12 protein in primary sperm-ZP binding. The protein is not connected to sperm motility and secondary sperm-ZP binding.
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Rosiglitazone Improves Insulin Resistance, Lipid Profile and Promotes Adiposity in a Genetic Model of Metabolic Syndrome X
O. ŠEDA, L. KAZDOVÁ, D. KŘENOVÁ, V. KŘEN
RSG is a member of the TZD group of drugs widely used in treatment of type 2 diabetes. The underlying mechanism of TZD action in insulin-sensitive tissues is not fully understood. In this study we show that 14-day RSG administration in a new rodent model of metabolic syndrome X, polydactylous rat strain (PD/Cub), substantially improves its lipid profile (serum TGs 4.20 ± 0.23 vs 2.34 ± 0.14 mmol/l, P < 0.0001; FFA 0.46 ± 0.05 vs 0.33 ± 0.02 mmol/l, P = 0.017), diminishes the liver TG depots (15.76 ± 0.60 vs 8.44 ± 0.55 ?mol/g, P < 0.0001), serum insulin concentrations (1.10 ± 0.08 vs 0.63 ± 0.02 nmol/l, P < 0.0001) and promotes visceral adiposity (adiposity index 1.28 ± 0.03 vs 1.85 ± 0.07, P < 0.0001). No changes were observed in serum or liver concentrations of cholesterol. Concomitantly, both basal and insulin-stimulated glycogen synthesis in red-fibre type muscle (m. soleus) was enhanced, as well as glucose uptake into adipose tissue. However, glucose oxidation in soleus (basal and insulin-stimulated) remained unchanged. In consent with previously published data we suggest the current pharmacogenetic study as a further proof of substantial influence of genetic background on the physiological outcome of TZD therapy.
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Short Communication
Freezing and Thawing of Murine Bone Marrow-Derived Dendritic Cells Does Not Alter Their Immunophenotype and Antigen Presentation Characteristics
L. MENDOZA, J. BUBENÍK, M. INDROVÁ, J. BIEBLOVÁ, V. VONKA, J. ŠÍMOVÁ
The aim of this paper was to assess whether the BMDC after freezing and thawing are capable to retain the immunophenotype and antigen-presenting capacity. BMDC were generated from bone marrow precursor cells as described previously by culturing the cells in medium containing GM-CSF and IL-4. Afterwards, the cells were harvested, counted and used for phenotyping and priming of syngeneic spleen cells. For cryopreservation, the BMDC were frozen in the presence of 10% of dimethylsulphoxide (DMSO) and 90% foetal calf serum. Forty to fifty percent of both samples, frozen/thawed as well as fresh BMDC, exhibited characteristic DC morphology, and the DC obtained from the frozen/thawed samples expressed a similar frequency of MHC class I-, MHC class II-, CD80-, CD86-, CD11c-, CD11b-, CD54- and CD205-positive cells as fresh DC. To examine the in vitro priming effect of cryopreserved BMDC on syngeneic non-adherent murine C57BL/6 (B6) spleen cells, the BMDC were thawed, pulsed with the lysate prepared from HPV 16-associated tumour MK16 and used for 3H-thymidine assay. The findings of the experiments indicate that fresh as well as cryopreserved murine BMDC preparations pulsed with tumour lysate were efficient to prime the mitogenic activity of syngeneic non-adherent splenocytes. Taken together, the results suggest that frozen/thawed BMDC are morphologically, phenotypically and functionally comparable with fresh BMDC and can be used for construction of dendritic cell-based tumour vaccines.
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DicodonUse: The Programme for Dicodon Bias Visualization in Prokaryotes
J. PAČES, V. PAČES
The DicodonUse programme is aimed at a fast and simple assessment of genes present in prokaryotic nucleotide sequences. It identifies open reading frames that are not genes, and it distinguishes the genes that inherently belong to the genome in question from the genes that were inserted into the genome in the course of evolution. The programme is based on frequencies of dicodons used by the organism.
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