Volume 60 (2014) Suppl. 1
Review Article
Frequent Chromatin Rearrangements in Myelodysplastic Syndromes – What Stands Behind?
(myelodysplastic syndromes / chromosomal rearrangements / chromosome 5 deletions / chromatin structure / architecture of the cell nucleus / chromothripsis)
E. Pagáčová1, M. Falk1, I. Falková1,2,3, E. Lukášová1, K. Michalová4, A. Oltová3, I. Raška5, S. Kozubek1
1Institute of Biophysics, Academy of Sciences of the Czech Republic, v. v. i., Brno, Czech Republic
2Department of Medical Technology, St. Elisabeth University of Health and Social Sciences, Bratislava, Slovak Republic, Signatory of Magnae Chartae Universitatum Bologna, European Union
3Department of Internal Medicine – Haematology and Oncology, University Hospital Brno and Faculty of Medicine, Masaryk University, Brno, Czech Republic
4Centre of Oncocytogenetics, General University Hospital in Prague, Prague, Czech Republic, and The Institute of Haematology and Blood Transfusion (IHBT), Prague, Czech Republic
5Institute of Cellular Biology and Pathology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
Pages: 1 - 7 (PDF)
Corresponding author: Martin Falk, Institute of Biophysics, Academy of Sciences of CR, v. v. i., Královopolská 135, 612 65 Brno, Czech Republic. Phones: (+420) 541515116/165; (mobile) (+420) 728084060; e-mails: mfalk@seznam.cz, falk@ibp.cz
Review Article
Novel Insights into the Electrochemical Detection of Nitric Oxide in Biological Systems
(nitric oxide / electrochemical detector / biological systems / carbon fibre)
M. Pekarová1, A. Lojek1, J. Hrbáč2, R. Kuchta3, J. Kadlec3, L. Kubala1
1Institute of Biophysics, Academy of Sciences of the Czech Republic, v. v. i., Brno, Czech Republic
2Department of Physical Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic
3Faculty of Electrical Engineering and Communication, Brno University of Technology, Brno, Czech Republic
Pages: 8 - 12 (PDF)
Corresponding author: Michaela Pekarová, Institute of Biophysics, Academy of Sciences of the Czech Republic, v. v. i., Královopolská 135, 612 65 Brno, Czech Republic. E-mail: pekarovam@ibp.cz
Review Article
Structural Basis of Polycomb Bodies
(Polycomb group proteins / Polycomb body / post-translational chromatin modifications / nuclear subcompartments / nuclear factories / chromatin domains)
J. ŠMIGOVÁ1, P. JUDA1, J. KREJČÍ2, I. RAŠKA1
1Institute of Cellular Biology and Pathology, First Faculty of Medicine, Charles University in Prague, Czech Republic
2Institute of Biophysics, Academy of Sciences of the Czech Republic, v. v. i., Brno, Czech Republic
Pages: 13 - 20 (PDF)
Corresponding author: Jana Šmigová; Institute of Cellular Biology and Pathology, First Faculty of Medicine, Charles University in Prague, Albertov 4, 12801 Prague 2, Czech Republic. Phone: (+420) 224 968 014; Fax: (+420) 224 917 418; email: jsmig@lf1.cuni.cz
Review Article
Recent Approaches in Tooth Engineering Research
(engineering / tooth / stem cells /culture techniques)
E. Švandová1,2, B. Veselá1,2, J. Křivánek3, A. Hampl3, E. Matalová1,4
1Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v. v. i., Brno, Czech Republic
2Department of Experimental Biology, Faculty of Science, 3Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
4Department of Physiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
Pages: 21 - 29 (PDF)
Corresponding author: Eva Švandová, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v. v. i., Veveří 97, 602 00 Brno, Czech Republic. Phone: (+420) 532 290 163; Fax: (+420) 541 212 988; e-mail: 184576@mail.muni.cz
Original Article
Properties of Neural Crest-Like Cells Differentiated from Human Embryonic Stem Cells
(stem cell differentiation / neural crest / odontogenesis / cell therapies)
J. KŘIVÁNEK1, E. ŠVANDOVÁ2, J. KRÁLIK1, Š. HAJDA1, R. FEDR4, V. VINARSKÝ1, J. JAROŠ1, K. SOUČEK3, 4, M. BUCHTOVÁ2, E. MATALOVÁ2, A. HAMPL1, 3
1Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno
2Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic
3International Clinical Research Centre, St. Anne’s University Hospital, Brno, Czech Republic
4Department of Cytokinetics, Institute of Biophysics AS CR, v. v. i., Brno, Czech Republic
Pages: 30 - 38 (PDF)
Corresponding author: Aleš Hampl, Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 126/3, 625 00 Brno, Czech Republic. Phone: (+420) 549 491 362; Fax: (+420) 549 498 001; e-mail: ahampl@med.muni.cz
Original Article
The Effects of Chloroquine and Hydroxychloroquine on Nitric Oxide Production in RAW 264.7 and Bone Marrow-Derived Macrophages
(chloroquine / cytotoxicity / hydroxychloroquine / macrophages / NO production)
T. Perečko1, R. B. Kassab1,2, O. Vašíček1, M. Pekarová1, V. Jančinová3, A. Lojek1
1Institute of Biophysics, Academy of Sciences of the Czech Republic, v. v. i., Brno, Czech Republic
2Zoology and Entomology Department, Faculty of Science, Helwan University, Ain Helwan, Cairo, Egypt
3Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovak Republic
Pages: 39 - 44 (PDF)
Corresponding author: Tomas Perecko, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, 612 65 Brno, Czech Republic. Phone: (+420) 541 517 263; Fax: (+420) 541 211 293; e-mail: tomas.perecko@ibp.cz
Original Article
Visualizing Stable Features in Live Cell Nucleus for Evaluation of the Cell Global Motion Compensation
(cell global motion compensation / UV laser bleaching / image registration / stable features / algorithm evaluation)
D. V. Sorokin1, J. Suchánková2, E. Bártová2, P. Matula1
1Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Brno, Czech Republic
2Institute of Biophysics, Academy of Sciences of the Czech Republic, v. v. i., Brno, Czech Republic
Pages: 45 - 49 (PDF)
Corresponding author: Dmitry V. Sorokin, Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Botanická 68a, 602 00, Brno, Czech Republic. Phone: (+420) 549 497 684; e-mail: sorokin@fi.muni.cz
Original Article
Utilization of an Automated Pipetting System in the Cell Line-Based Screening of the Activity of a DNA-Damaging Anti-Tumour Drug
(cell line screen / drug treatment / liquid handling station / epMotion®)
T. SUCHÁNKOVÁ1, P. OVESNÁ2, P. SAMADDER3, K. SOUČEK1, 4
1Institute of Biophysics, Academy of Sciences of the Czech Republic, v. v. i., Brno, Czech Republic
2Institute of Biostatistics and Analyses, Masaryk University, Brno, Czech Republic
3National Centre for Biomolecular Research, Faculty of Science and Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
4Centre of Biomolecular and Cellular Engineering, International Clinical Research Centre, St. Anne’s University Hospital Brno, Brno, Czech Republic
Pages: 50 - 55 (PDF)
Corresponding author: Tereza Suchánková and Karel Souček, Institute of Biophysics, Academy of Sciences of the Czech Republic, v. v. i., Královopolská 135, 61265 Brno, Czech Republic. e-mails: suchankovatereza@ibp.cz; ksoucek@ibp.cz.
Short Communication
Phthalates Deregulate Cell Proliferation, but Not Neuroendocrine Transdifferentiation, in Human LNCaP Prostate Cancer Cell Model
(phthalates / prostate cancer cells / cell cycle modulation / neuroendocrine transdifferentiation / androgen receptor)
E. HRUBÁ1, Z. PERNICOVÁ1, 2, L. PÁLKOVÁ3, K. SOUČEK1, 2, J. VONDRÁČEK1, M. MACHALA3
1Department of Cytokinetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v. v. i., Brno, Czech Republic
2Center of Biomolecular and Cellular Engineering, International Clinical Research Center, St. Anne’s University Hospital Brno, Brno, Czech Republic
3Department of Chemistry and Toxicology, Veterinary Research Institute, Brno, Czech Republic
Pages: 56 - 61 (PDF)
Corresponding author: Jan Vondráček, Institute of Biophysics AS CR, Královopolská 135, 612 65 Brno, Czech Republic. Phone: (+420) 541 517 168; Fax: (+420) 541 211 293; e-mail: vondracek@ibp.cz
Short Communication
Three-Dimensional Reconstruction of the S885A Mutant of Human Mitochondrial Lon Protease
(transmission electron microscopy / 3D reconstruction / AAA+ protease / human mitochondrial protein)
S. KEREÏCHE1, L. KOVÁČIK1, V. PEVALA2, L. AMBRO2, J. BELLOVÁ2, E. KUTEJOVÁ2, 3, I. RAŠKA1
1Institute of Cellular Biology and Pathology, First Faculty of Medicine, Charles University in Prague, Czech Republic
2Department of Biochemistry and Structural Biology, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia
3Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Pages: 62 - 65 (PDF)
Corresponding author: Sami Kereïche, Institute of Cellular Biology and Pathology, First Faculty of Medicine, Charles University in Prague, Albertov 4, 128 01 Prague 2, Czech Republic. Phone: (+420) 224 968 000; Fax: (+420) 224 917 418; e-mail:skere@lf1.cuni.cz
Short Communication
Sub-Volume Averaging of Repetitive Structural Features in Angularly Filtered Electron Tomographic Reconstructions
(electron tomography / missing wedge / limited specimen tilting / sub-volume averaging)
L. KOVÁČIK1, S. KEREÏCHE1, P. MATULA2, I. RAŠKA1
1Institute of Cellular Biology and Pathology, First Faculty of Medicine, Charles University in Prague, Czech Republic
2Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Brno, Czech Republic
Pages: 64 - 70 (PDF)
Corresponding author: Lubomír Kováčik, ICBP, First Faculty of Medicine, Charles University in Prague, Albertov 4, 128 01 Prague 2, Czech Republic. Phone: (+420) 224 968 000; Fax: (+420) 224 917 418; e-mail: lubomir.kovacik@lf1.cuni.cz
Short Communication
Mysterious Role of H3K56ac in Embryonic Stem Cells
(H3K56ac / p300/CBP / pluripotency / chromatin)
S. STEJSKAL, L. TESAŘOVÁ, I. KOUTNÁ
Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Brno, Czech Republic
Pages: 71 - 75 (PDF)
Corresponding author: Stanislav Stejskal, Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Botanická 68a, 602 00 Brno, Czech Republic. Phone: (+420) 549 483 976; fax: (+420) 549 498 360; email: darkelf@mail.muni.cz
Short Communication
Advanced Microscopy Techniques Used for Comparison of UVA- and γ-Irradiation-Induced DNA Damage in the Cell Nucleus and Nucleolus
(UVA / γ-irradiation / DNA damage response / nucleolus / living cell studies)
L. Stixová1, T. Hrušková1, P. Sehnalová1, S. Legartová1, S. Svidenská2, S. Kozubek1, E. Bártová1
1Institute of Biophysics, Academy of Sciences of the Czech Republic, v. v. i., Brno, Czech Republic
2Institute of Cellular Biology and Pathology, First Faculty of Medicine, Charles University in Prague, Czech Republic
Pages: 76 - 84 (PDF)
Corresponding author: Lenka Stixová, Institute of Biophysics, Academy of Sciences of the Czech Republic, v. v. i., Královopolská 135, 612 65, Brno, Czech Republic. Phone: (+420) 541 517 141; Fax: (+420) 541240498; e-mail: lenka@ibp.cz
Short Communication
Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods
(hiPSCs / lentivirus / Sendai virus / episomal reprogramming)
P. ŠIMARA1,2, L. TESAŘOVÁ1, S. PAĎOUROVÁ1,2, I. KOUTNÁ1,2
1Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Brno, Czech Republic
2International Clinical Research Center, St. Anne’s University Hospital Brno, Czech Republic
Pages: 85 - 89 (PDF)
Corresponding author: Pavel Šimara, Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic. Phone: (+420) 549 493 976; Fax: (+420) 549 493 976; e-mail: p.simara@mail.muni.cz
Short Communication
Haematopoietic Developmental Potential of Human Pluripotent Stem Cell Lines
(human pluripotent stem cells / haematopoietic development / embryoid bodies / CD34+ cells)
L. TESAŘOVÁ1, P. ŠIMARA1, 2, S. STEJSKAL1, I. KOUTNÁ1, 2
1Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Brno, Czech Republic
2International Clinical Research Center, St. Anne’s University Hospital Brno, Czech Republic
Pages: 90 - 94 (PDF)
Corresponding author: Lenka Tesařová, Faculty of Informatics, Masaryk University, Botanická 68a, 602 00 Brno, Czech Republic. Phone: (+420) 549 493 976; Fax: (+420) 549 498 360; e-mail: cbia.muni@gmail.com.
