Volume 44(1998) No. 3
AD MULTOS ANNOS "Be at the nation's service"1
My Personal Recollections of Radslav Kinsky's Immunological Career on the Occasion of His 70th Birthday
I. HILGERT.....................................................75
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Corresponding author: Ivan Hilgert, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic. Vídeòská 1083, 142 20 Prague, Czech Republic.
Review
A Personal Short History of Some Concepts of the Foeto-Maternal Relationship
G. CHAOUAT.....................................................81
U 131 INSERM, Hôpital A. Béclære, Clamart, France
Corresponding author: George Chaouat, U 131 Inserm, Hôpital A. Béclære, 92141 Clamart, CEDEX France.@LH 6
Articles
Antibody against 28-kDA Intra-acrosomal Sperm Protein as a Tool for Evaluation of Acrosomal Integrity in Bull Spermatozoa
J. PÌKNICOVÁ1, J. MOOS1,2.....................................93
1Department of Biology and Biochemistry of Fertilization, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague , Czech Republic 2Sigma-Aldrich, Prague, Czech Republic@LH 6
Corresponding author: Jana Pìknicová, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Vídeòská 1083, 142 20 Prague 4, Czech Republic. Tel. 420 2 475 2642; Fax 420 2 471 34 43; e-mail:jpeknic@biomed.cas.cz.
Abstract
A Possible Cross-talk of Retinoic Acid- and TPA-Driven Myeloid Differentiation Pathways
K. ZEMANOVÁ, J. ŠMARDA.........................................97
Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Brno, Czech Republic
Corresponding author: Jan Šmarda, Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Kotláøská 2, 611 37 Brno, Czech Republic. Tel. (420)-5-41129538 Fax (420)-5-41211214; e-mail smarda@sci.muni.cz.
Abstract.
Linkage Mapping of the Interleukin 1Ð Converting Enzyme (Il1bc) and the Glutamate Receptor Subunit KA1 (Grik4) Genes to Rat Chromosome 8
M. PRAVENEC1,2, V. KØEN1,2, M. HOPE3, J.-M. WANG3, E. ST. LEZIN3........................................................107
1Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
2Institute of Biology, 1st Medical Faculty, Charles University, Prague, Czech Republic 3Department of Laboratory Medicine, University of California, San Francisco, USA Corresponding author: Michal Pravenec, Institute of Physiology, Academy of Sciences of the Czech Republic, Vídeòská 1083, 142 20 Prague 4, Czech Republic. Tel./Fax: (4202) 475 2297; e-mail: pravenec@biomed.cas.cz.
Abstract.
Effect of pH on Proteinase Secretion by Transformed Fibroblast Populations
J. CHALOUPKA1, H. KUÈEROVÁ1, L. VÁCHOVÁ1, E. KRCHÒÁKOVÁ2, A. CHALOUPKOVÁ2, L. PAVLÍKOVÁ2, D. ZICHA3, P. VESELÝ2.........111 1Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
2Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
3MRC Muscle and Cell Motility Unit, King's College London, London, UK Corresponding author: Pavel Veselý, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague 7 6, Flemingovo nám. 2, CZ-16637, Czech Republic.
Abstract.
Short Communication
5 Cellular Activity of Murine Phagocytes Isolated from Peripheral Blood by a Discontinuous Gradient
M. A. de PABLO, C. ALVAREZ, A. M. GALLEGO, E. ORTEGA, P. L. PANCORBO, G. ALVAREZ de CIENFUEGOS LÓPEZ......................117 Department of Health Sciences, Faculty of Experimental Sciences, University of Jaén, Jaén, Spain
Corresponding author: Gerardo Alvarez de Cienfuegos López, Department of Health Sciences, University of Jaén, 23071 Jaén, Spain. Tel. +34-53-212164; Fax +34-53-212141; e-mail gcienfue@ujaen.es.
Abstract.
Antibody against 28-kDA Intra-acrosomal Sperm Protein as a Tool for Evaluation of Acrosomal Integrity in Bull Spermatozoa
J. PÌKNICOVÁ, J. MOOS
Monoclonal antibody ACR.4 recognizing specifically the 28-kDa intra-acrosomal protein was prepared by immunization of mice with acetic acid extract of boar spermatozoa, but cross-reacted also with bull intra-acrosomal protein. This monoclonal antibody was used for immunostaining analysis of bull spermatozoa before and during capacitation and ionophore-induced acrosome reaction. Immunostaining analysis showed changes of 28-kDa protein in the acrosome during capacitation and loss of this protein after induced acrosome reaction by ionophore A23187. Therefore, this monoclonal antibody can be used in the bull spermatozoa as immunological test for detection of the acrosome state after manipulation with spermatozoa or after freezing/thawing. This test could be useful (apart from morphology and motility) for the selection of suitable spermatozoa for insemination or in vitro fertilization.
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A Possible Cross-talk of Retinoic Acid- and TPA-Driven Myeloid Differentiation Pathways
K. ZEMANOVÁ, J. ŠMARDA
Retinoic acid (RA) and phorbol esters are important regulators of cellular proliferation and differentiation processes which are capable of arresting growth and inducing differentiation of various leukemic cells. We have studied and compared the effects of these agents on the line of v-myb oncogene-transformed chicken monoblasts BM2. We found that although there are differences in the differentiation kinetics and cell cycle stop points, the differentiation pathways induced by RA and phorbol esters are significantly interconnected.
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Linkage Mapping of the Interleukin 1Ð Converting Enzyme (Il1bc) and the Glutamate Receptor Subunit KA1 (Grik4) Genes to Rat Chromosome 8
M. PRAVENEC1,2, V. KØEN1,2, M. HOPE3, J.-M. WANG3, E. ST. LEZIN3
Genes for interleukin 1Ð converting enzyme (Il1bc) and the glutamate receptor subunit KA1 (Grik4) have been mapped to a centromeric region of rat chromosome 8 using linkage analysis of HXB and BXH recombinant inbred strains. The current results demonstrate that rat chromosome 8 is largely homologous to mouse chromosome 9.
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Effect of pH on Proteinase Secretion by Transformed Fibroblast Populations
J. CHALOUPKA1, H. KUÈEROVÁ1, L. VÁCHOVÁ1, E. KRCHÒÁKOVÁ2, A. CHALOUPKOVÁ2, L. PAVLÍKOVÁ2, D. ZICHA3, P. VESELÝ2
Effect of pH on secretion of proteolytic enzymes in cell cultures of three clonal lines of transformed fibroblasts (K2, T15 and K4) was studied by using 14C-labelled denatured proteins as substrate. One line of malignant macrophages derived from mouse reticulum cell sarcoma (J774.1) was used for comparison. The relative motility index of all cell lines was derived by computer analysis of quantitative estimations of cell dispersion in single-cell-derived colonies. Cultivation at pH 6.5 decreased the growth rate in most experiments as compared with that at pH 7.4, and stimulated cell motility to a different extent. The population of mouse malignant macrophages produced several-fold higher extracellular proteolytic activity than the fibroblast lines. Secretion of proteinases by the malignant macrophages was significantly stimulated by the lower pH. Enzyme secretion by two of the three fibroblast derivatives was also stimulated by acidic pH but to a lesser extent than the secretion of the malignant macrophages. The assessment of motility done by measurement of dispersion of cells in colony proved a positive correlation between motility and proteinase secretion in J774.1 cells and one transformed fibroblast clone (T15) but 7 not in the two other clonal lines.
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Short Communication
5 Cellular Activity of Murine Phagocytes Isolated from Peripheral Blood by a Discontinuous Gradient
M. A. de PABLO, C. ALVAREZ, A. M. GALLEGO, E. ORTEGA, P. L. PANCORBO, G. ALVAREZ de CIENFUEGOS LÓPEZ.
A mixture of Ficoll 400 and sodium diatrizoate (Hypaque) at a density of 1.077 g/ml has been used to isolate the mononuclear cells from the remaining haematic cells. A simple, inexpensive and classical method was established to obtain substantially erythrocyte-free polymorphonuclear cell preparations from mouse peripheral blood, using a mixture of the same substances but at a density of 1.119 g/ml. This method along with that at a density of 1.077 g/ml allows two cellular bands to appear which contain mononuclear and polymorphonuclear (PMN) cells, respectively. Using this method, the counts of monocytes isolated from peripheral blood are significantly greater than those obtained by a one-step Ficoll-Hypaque procedure. On the contrary, the counts of PMN cells are significantly smaller than when sedimentation in dextran (6% solution) is used after gradient centrifugation. In this paper, chemiluminescence assay has been used to analyze the possible variations in phagocytic activity of cells isolated by both procedures, since it appears to be one of the most sensitive methods available for this purpose. The results obtained show a slightly greater activation in monocytes and PMN cells isolated by one-step Ficoll-Hypaque procedure, in comparison with another method which uses both Ficoll-Hypaque 1077 and Ficoll-Hypaque 1119, although statistical differences were not significant.
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