Volume 46 (2000) No. 4
Volume 46 (2000) No. 4
Mycobacterium tuberculosis and Human Macrophage: the Bacillus with "Environment-Sensing"
M. FRAZIANO1, V. COLIZZI1, F. MARIANI2.................................127
1Experimental Medicine Institute-Rome, National Council of Research, Rome, Italy
2Department of Biology, University of Rome "Tor Vergata", Rome, Italy
Corresponding author: Francesca Mariani, Experimental Medicine Institute-Rome, National Council of Research, Via del Fosso del Cavaliere 100, 00133 Rome, Italy. Tel.: 39 06 4993 4206;
Fax: 39 06 4993 4257; e-mail: Francesca.Mariani@ims.rm.cnr.it.
No abstract available.
Full text. 127-128 129-130
Articles
Genes Involved in the Destruction of Leukaemic Cells by Induced Photosensitivity
M. BELIČKOVÁ, H. BRUCHOVÁ, H. CAJTHAMLOVÁ, Z. HRKAL, R. BRDIČKA...........131
Institute of Haematology and Blood Transfusion, Prague
Corresponding author: Radim Brdička, Institute of Hematology and Blood Transfusion, U Nemocnice 1,
128 20 Prague 2, Czech Republic. Fax: 420 (2) 21977371; e-mail: molgen@uhkt.cz.
Abstract.
Full text. 131-132 133-134 135
Ultrastructural Analysis of v-myb Oncogene Product Cooperation with Components of Avian
Cell Nuclear Matrix
J. KORB, J. ŠTOKROVÁ, V. KARAFIÁT............................................137
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Corresponding author: Jan Korb, Institute of Molecular Genetics, Academy of Sciences of
the Czech Republic, Flemingovo nám. 2, 166 37 Prague 6, Czech Republic.
Abstract.
Full text. 137-138 139 140 141-142
Aggregated and Monomeric Forms of Proteins in Boar Seminal Plasma: Characterization and Binding Properties
P. MAŇÁSKOVÁ1,2, J. LIBERDA2, M. TICHÁ2, V. JONÁKOVÁ1...........................143
1Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
2Department of Biochemistry, Charles University, Prague, Czech Republic
Corresponding author: Věra Jonáková, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 37 Prague 6, Czech Republic. Fax: 420 (2) 24310955; Tel: 420 (2) 20183347; e-mail: vjon@img.cas.cz.
Abstract.
Full text. 143-144 145 146-147 148-149 150-151
Short Communications
HLA-A, HLA-B and HLA-C Polymorphism in the Slovak Population
E. KULCSÁROVÁ1, M. BUC1, S. FERENČÍK2..........................153
1Department of Immunology, School of Medicine, Comenius University, Bratislava, Slovakia
2Institute of Immunology, University Hospital, Essen, Germany
Corresponding author: Enikö Kulcsárová, Department of Immunology, Faculty of Medicine,
Sasinkova 4, 813 72 Bratislava, Slovakia. Tel.: +421 7 59357450; Fax: +421 7 59357578; e-mail:kulcsarova@hotmail.com.
Abstract.
Full text. 153-154 155-156
Tissue-Engineered Skin in the Treatment of Vitiligo Lesions
P. ARENBERGER1, L. BROŽ2, P. VESELÝ3, B. HAVLÍČKOVÁ1,
E. MATOUŠKOVÁ3.......................................157
1Department of Dermatology, 3rd Medical Faculty, Charles University Hospital, Prague, Czech Republic
2Prague Burn Centre, 3rd Medical Faculty, Charles University, Prague, Czech Republic
3Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Corresponding author: Petr Arenberger, Department of Dermatology, Charles University Hospital, Šrobárova 50, 100 34 Prague 10, Czech Republic. Tel.: +420 (2) 6716 2354; Fax.: +420 (2) 9002 4343.
Abstract.
Full text. 157 158 159-160
Articles
Genes Involved in the Destruction of Leukaemic Cells by Induced Photosensitivity
M. BELIČKOVÁ, H. BRUCHOVÁ, H. CAJTHAMLOVÁ, Z. HRKAL, R. BRDIČKA
Gene expression changes were observed in the HEL and HL-60 cell lines after the stimulation of protoporphyrin IX synthesis by ALA administration and photodynamic process induction. Isolated
ribonucleic acids were radiolabelled by reverse transcription, and the cDNA obtained was hybridized to membrane macroarrays (Clontech 7742-1) containing 588 gene probes. Besides changes in the activity of genes supposed to be involved in the programmed cell death and DNA reparation processes, increased or diminished transcription activity was also observed in several other genes; the reason for this phenomenon was not clear. The activation of programmed-cell-death genes appeared after the ALA load application, indicating the toxic effect of ALA. The gene expression changes observed in the two cell lines differed substantially, only a few of them were common for both cell lines.
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Ultrastructural Analysis of v-myb Oncogene Product Cooperation with Components of Avian
Cell Nuclear Matrix
J. KORB, J. ŠTOKROVÁ, V. KARAFIÁT
The cooperation of the v-Myb oncoprotein with extracted nuclear matrix of avian haematopoietic cells expressing the v-myb oncogene was studied by means of immunoelectron microscopy. The nuclear matrix
was extracted by a gentle method of detergent treatment at moderate ionic strength and visualized either in ultrathin LR White sections, in unembedded resin-free sections, and in addition by the aqueous spreading technique. Using anti-Myb polyclonal antibody we have shown interaction of the v-Myb protein product with extracted nuclear matrix. This oncoprotein, however, was easily released from the structure by a detergent as well as by DNAase treatment and ammonium sulphate extraction. Prefixation of structures before detergent treatment prevented this extraction. The v-Myb protein marker was distributed in clusters or associated with fibrillar structures in most cases. Single markers decorating these fibrilllar or less dense structures were also detected.
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Aggregated and Monomeric Forms of Proteins in Boar Seminal Plasma: Characterization and Binding Properties
P. MAŇÁSKOVÁ, J. LIBERDA, M. TICHÁ, V. JONÁKOVÁ
Boar seminal plasma was separated into five protein fractions (I-V) (>100, 55, 45, 30, 5-15 kDa) by gel filtration chromatography on Sephadex G-75 SF at pH 7.4. RP HPLC of protein fractions I-V and N-terminal sequencing of their individual components revealed that the high-molecular-weight aggregates consisted mainly of DQH sperm surface protein and AQN, AWN, PSP II spermadhesins, while the fraction IV consisted of heterodimers of PSP spermadhesins only. Spermadhesins as monomers were present in seminal plasma in a very low amount. Biotinylated fractions I-IV containing AWN, AQN, DQH, and PSP proteins were bound to boar epididymal and ejaculated spermatozoa with the same efficiency. Aggregates containing AWN, AQN, DQH, PSP II proteins (fractions I-III) and their HPLC-separated monomeric forms interacted with phosphorylcholine. Aggregates containing the DQH protein and AWN spermadhesins as well as their separated monomeric proteins interacted strongly with acidic polysaccharides. PSP II interacted with some acidic polysaccharides, while the fraction IV corresponding to heterodimer PSP I/PSP II did not show any binding to acidic polysaccharides and zona pellucida. Fractions I-III showed affinity to cholesterol. The strongest interaction was observed between biotinylated glycoproteins of porcine zona pellucida and AWN 1-containing aggregates and separated proteins. AQN 1 spermadhesin efectivelly blocked the sperm binding to oocytes. These results suggest that under physiological conditions, the aggregated forms of seminal plasma proteins (DQH, AQN, AWN, PSP II) rather than the individual proteins might take part in coating the sperm surface, in sperm capacitation and in primary binding of spermatozoa to zona pellucida of the ovum.
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HLA-A, HLA-B and HLA-C Polymorphism in the Slovak Population
E. KULCSÁROVÁ, M. BUC, S. FERENČÍK
The occurrence rates of class I HLA alleles were investigated in a sample of the Slovak population by a PCR-SSP method. The frequencies of HLA-A alleles ranged from 0.00 for A*4301 to 0.2798 for A*201-22; the frequencies of HLA-B alleles ranged from 0.00 for B*4601, B*4801-3, B*5901, B*7301, and B*8101 to 0.1101 for B*4402-10, and those of HLA-C alleles from 0.00 for *Cw1301 and *Cw1402-3 to 0.2661 for *Cw0701-10. The occurrence rates of class I HLA alleles established in our study were compared with those in the Czech population. No significant differences were found.
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P. ARENBERGER, L. BROŽ, P. VESELÝ, B. HAVLÍČKOVÁ,
E. MATOUŠKOVÁ
Vitiligo is characterized by the loss of skin pigmentation due to the destruction of melanocytes. Its treatment is usually difficult. For stable cases, melanocyte transplantation is the method of choice. A newly developed treatment with recombined human/porcine skin methodology, permitting easy
handling of the graft is described in the present work. In five vitiligo patients, autologous epidermal
cells were obtained from pigmented thin skin biopsies. The cells were cultured on a dried cell-free porcine dermis with the 3T3 feeder layer technique. After 10 days melanocytes were regularly dispersed in confluent keratinocyte cultures. Upside-down delivery of epidermal cells was used. The epidermal layer was directly applied onto a dermabraded vitiligo lesion with porcine dermis covering the lesion. Pigmentation started to be visible 4-6 weeks after grafting. After using the above described methodology, the pigmentation appeared in the range of 65-80% of the grafted area. Additional UVA irradiation enhanced the treatment success up to 100%. The surgical vitiligo treatment appears to be a reasonable method of choice in stable vitiligo cases of a disease lasting for at least two years, which means for approximately 5% of all vitiligo patients.
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