Volume 51 (2005) No. 1
MHC Class I Downregulation, Tumour Escape from Immune Surveillance and Design of Therapeutic Strategies
J. BUBENÍK.........................................................................1
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Corresponding author: Jan Bubeník, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic,
Flemingovo nám. 2, 166 37 Praha 6, Czech Republic. Tel.: +420 220 183 234; Fax: +420 224 310 955; e-mail: bubenik@img.cas.cz
No abstract available.
Full text. 1-2
Original Articles
Expression of CD44v6 Correlates with Cell Proliferation and Cellular Atypia in Urothelial Carcinoma
Cell Lines 5637 and HT1197
J. KUNCOVÁ1, Z. KOSTROUCH2, M. VIALE3, R. REVOLTELLA4, V. MANDYS5.................3
1Clinical Department of Urology, 3rd Faculty of Medicine, Charles University, Prague, Czech Republic
2Institute of Inherited Metabolic Disorders, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
3National Cancer Research Institute, Pharmacotoxicology Unit, Genova, Italy
4Institute of Biomedical Technologies, Immunobiology and Cell Differentiation Unit, CNR, Pisa, Italy
5Department of Pathology, 3rd Faculty of Medicine, Charles University, Prague, Czech Republic
Corresponding author: Václav Mandys, Department of Pathology, 3rd Faculty of Medicine, Charles University in Prague,
Šrobárova 50, 100 34, Prague 10, Czech Republic. Tel.: (+420) 267 162 500; fax: (+420) 267 163 002; e-mail: mandys@fnkv. cz
Abstract.
Full text. 3-11
Characteristic of Two Mouse bcr-abl-Transformed Cell Lines: I. General Properties of the Cells
E. SOBOTKOVÁ1, V. LUDVÍKOVÁ1, M. PETRÁČKOVÁ1, M. DUŠKOVÁ1, K. SMETANA2, F. JELÍNEK1, I. MARINOV3, V. VONKA1...................................................................................................12
1Department of Experimental Virology, Institute of Hematology and Blood Transfusion, 2Clinical Department, Institute of Hematology
and Blood Transfusion, 3Laboratory of Flow Cytometry, Institute of Hematology and Blood Transfusion, Prague, Czech Republic
Corresponding author: Vladimír Vonka, Institute of Hematology and Blood Transfusion, Department of Experimental Virology,
U Nemocnice 1, 128 20 Praha 2. Fax: (+420) 221 977 392; e-mail: vladimir.vonka@uhkt.cz
Abstract.
Full text. 12-18
Immunotherapeutic Efficacy of Vaccines Generated by Fusion of Dendritic Cells and HPV16-Associated Tumour Cells
J. ŠÍMOVÁ, J. BUBENÍK, J. BIEBLOVÁ, M. INDROVÁ, T. JANDLOVÁ......................................................19
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Corresponding author: Jana Šímová, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nám. 2,
166 37 Prague 6, Czech Republic.
Abstract.
Full text. 19-24
Nucleoli in Human Early Erythroblasts (K2, K1, K1/2 cells)
K. SMETANA, I. JIRÁSKOVÁ, H. KLAMOVÁ..................................................................25
Clinical Department, Institute of Hematology and Blood Transfusion, Prague, Czech Republic
Corresponding author: Karel Smetana, Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague 2, Czech Republic.
Fax: (+ 420) 221 977 249; e-mail: karel.smetana@uhkt.cz
Abstract.
Full text. 25-28
Expression of CD44v6 Correlates with Cell Proliferation and Cellular Atypia in Urothelial Carcinoma
Cell Lines 5637 and HT1197
J. KUNCOVÁ, Z. KOSTROUCH, M. VIALE, R. REVOLTELLA, V. MANDYS
CD44 comprises a family of membrane adhesion molecules encoded by a single gene and diversified by alternative splicing and extensive posttranslational modifications. Alterations of CD44 expression patterns are linked to tumour invasion and formation of metastases. However, CD44 expression and its relation to the biological properties of tumours varies depending on the tumour type and origin. In transitional cell carcinoma of the urinary bladder, low CD44 expression is linked to enhanced tumor aggressiveness. We studied CD44 expression in two urothelial cancer cell lines, HT1197 and 5637. CD44s and a v6 variable exon-containing splice variants were detected in both cell lines by reverse transcription-PCR and by commercially available monoclonal antibodies. In both cell lines, Western blot analysis detected immunoreactive proteins with approximate size 70–85 kD, 95–110 kD, and 120–140 kD with CD44v6 antibody and weak bands with size 70–98 kD with CD44s antibody. At the cellular level, the pattern of CD44 immunoreactivity correlated with a lower level of cell differentiation and a higher degree of cell proliferation. In HT1197 cells, the CD44v6 was detected predominantly in small proliferating cells and in large multinuclear atypical cells. CD44s and CD44v6 displayed low immunoreactivity in HT1197 cells with a higher degree of epithelial differentiation. The 5637 cells expressed CD44v6 strongly and CD44s weakly. We conclude that CD44v6 expression correlates with a higher proliferative activity and with a stem cell-like phenotype in both cell lines and with cellular atypia in HT1197 cells.
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E. SOBOTKOVÁ, V. LUDVÍKOVÁ, M. PETRÁČKOVÁ, M. DUŠKOVÁ, K. SMETANA, F. JELÍNEK, I. MARINOV,
V. VONKA
we examined the properties of two mouse (Balb/c) established cell lines that express the Bcr-Abl protein and are oncogenic for syngeneic animals. Under standard conditions the two cell lines, viz. Ba-p210 (B210) and 12B1, expressed comparable amounts of the Bcr-Abl protein. However, they differed in a number of characteristics. From the morphological point of view, B210 cells were the more homogeneous, being mainly represented by leukaemic blastic cells with a large number of AgNORs as markers indicating a high proliferative activity. 12B1 cells were more polymorphic and giant cells were detected within their populations. Many 12B1 cells exhibited nuclear segmentation and “band-like“ structures. Markers of proliferation were less frequent in 12B1 and the tendency for aging was more pronounced in these cells. The 12B1 cells were slightly more sensitive to imatinib mesylate than B210 cells. In B210 cells, the expression of MHC class I was downregulated, which was not the case with 12B1 cells. Both cell lines induced leukaemia-like disease in mice after intravenous application but, as compared with B210, 12B1 cells were about 100 times more oncogenic and the disease they induced was more aggressive. Moreover, 12B1, but not B210, induced tumours after subcutaneous or intraperitoneal inoculation.
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J. ŠÍMOVÁ, J. BUBENÍK, J. BIEBLOVÁ, M. INDROVÁ, T. JANDLOVÁ
Utilization of vaccines generated by fusion of dendritic cells and tumour cells is a promising approach to tumour immunotherapy. We have examined the therapeutic efficacy of vaccines generated by fusion of HPV16-associated tumour cells TC-1 with syngeneic and allogeneic dendritic cells. Locally administered hybrid cells generated by fusion of MHC class I+ TC‑1 cells and syngeneic DC inhibited the growth of MHC class I+ TC-1 tumours, but not the growth of MHC class I- TC-1/A9-derived tumours. The growth of TC-1 tumours was also inhibited by hybrids generated by fusion of TC-1 cells and allogeneic DC. The therapeutic efficacy was enhanced by co-administration of the vaccine with synthetic immunostimulatory ODN CpG 1826.
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K. SMETANA, I. JIRÁSKOVÁ, H. KLAMOVÁ
Human early erythroid precursors classified according to the nuclear size were studied to provide information on nucleoli in these cells using simple cytochemical procedures for demonstration of RNA and proteins of silver-stained nucleolar organizers. K2 cells with nuclear diameter larger than 13 µm and K1 cells with nuclear diameter larger than 9 µm corresponding to proerythroblasts and macroblasts (large basophilic erythroblasts) mostly possessed large irregularly shaped nucleoli with multiple fibrillar centres representing “active nucleoli”. K1/2 cells with nuclear diameter smaller than 9 µm corresponding to small basophilic erythroblasts were usually characterized by the presence of micronucleoli representing “inactive nucleolar types”. On the other hand, a few K1/2 cells contained large nucleoli with multiple fibrillar centres similar to those present in K2 cells and thus appeared as “microproerythroblasts”. The nucleolar asynchrony expressed by the presence of large irregularly shaped nucleoli with multiple nucleoli (active nucleoli) and ring-shaped nucleoli (resting nucleoli) in one and the same nucleus of K2 or K1 cells was not exceptional and might reflect a larger resistance of these cells to negative factors influencing the erythropoiesis. The intranucleolar translocation of silver-stained nucleolus organized regions was noted in K2 cells and might indicate the premature aging of these cells without further differentiation. More studies, however, are required in this direction.
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