Volume 65 (2019) No. 3

Original Article

Reprogramming of Human Pancreatic Organoid Cells into Insulin-Producing β-Like Cells by Small Molecules and in Vitro Transcribed Modified mRNA Encoding Neurogenin 3 Transcription Factor

(diabetes / organoid / reprogramming / synthetic mRNA / in vitro transcription / IVT / neurogenin 3 / β-cell /
insulin / TGF / RepSox / small molecules)

T. KOBLAS1,3, I. LEONTOVYC1,3, S. LOUKOTOVA3, F. SAUDEK2

1Department of Experimental Medicine, 2Department of Diabetes, Institute for Clinical and Experimental
Medicine, Prague, Czech Republic

3First Faculty of Medicine, Charles University, Prague, Czech Republic

Pages: 109 - 123 (PDF)

Corresponding author: Tomas Koblas, Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, Prague, Czech Republic. Phone: (+420) 261 365 447; Fax: (+420) 261 362 820; e-mail: tomas.koblas@ikem.cz


Original Article

Enzymatic Isolation, Amplification and Characterization of Dental Pulp Stem Cells

(enzymatic digestion / dental pulp stem cells / trypsin / isolation)

N. PILBAUEROVA1, T. SOUKUP2, T. SUCHANKOVA KLEPLOVA1, J. SUCHANEK1

1Department of Dentistry, 2Department of Histology and Embryology, Charles University – Faculty of
Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic

Pages: 124 - 133 (PDF)

Corresponding author: Nela Pilbauerova, Department of Dentistry, University Hospital Hradec Králové, Sokolská 581, 500 05 Hradec Králové. Phone: (+420) 777245459; e-mail: nela.pilbauerova@lfhk.cuni.cz.


Original Article

Review of SRD5A3 Disease-Causing Sequence Variants and Ocular Findings in Steroid 5α-Reductase Type 3 Congenital Disorder of Glycosylation, and a Detailed New Case

(retinal dystrophy / SRD5A3-CDG / SRD5A3 / novel variant)

B. KOUSAL1,2, T. HONZÍK2, H. HANSÍKOVÁ2, N. ONDRUŠKOVÁ2, A. ČECHOVÁ2, M. TESAŘOVÁ2, V. STRÁNECKÝ2, M. MELIŠKA1, M. MICHAELIDES3,4, P. LIŠKOVÁ1,2

1Department of Ophthalmology, 2Department of Paediatrics and Adolescent Medicine, First Faculty of
Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic

3Moorfields Eye Hospital NHS Foundation Trust, London, United Kingdom

4UCL Institute of Ophthalmology, University College London, London, United Kingdom

Pages: 134 - 141 (PDF)

Corresponding author: Petra Liskova, Department of Paediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 2, 128 08 Prague 2, Czech Republic. Phone: (+420) 224 967 139; e-mail: petra.liskova@lf1.cuni.cz


Original Article

Deregulation of Selected MicroRNAs in Sinonasal Squamous Cell Carcinoma: Searching for Potential Prognostic Biomarkers

(biomarker / epigenetics / head and neck cancer / microRNA / sinonasal squamous cell carcinoma)

H. KOVAŘÍKOVÁ1, I. BARANOVÁ1, J. LACO2, K. ROZKOŠOVÁ2, H. VOŠMIKOVÁ2, M. VOŠMIK3, P. DUNDR4, K. NĚMEJCOVÁ4, J. MICHÁLEK5, V. PALIČKA1, M. CHMELAŘOVÁ1

1Institute of Clinical Biochemistry and Diagnostics, 2The Fingerland Department of Pathology, 3Department
of Oncology and Radiotherapy, Charles University, Faculty of Medicine in Hradec Králové and University
Hospital Hradec Králové, Czech Republic

4Department of Pathology, Charles University, First Faculty of Medicine and General University Hospital in
Prague, Czech Republic

5Department of Clinical and Molecular Pathology, Palacky University Olomouc, Faculty of Medicine and
Dentistry and University Hospital Olomouc, Czech Republic

Pages: 142 - 151 (PDF)

Corresponding author: Helena Kovaříková, Institute of Clinical Biochemistry and Diagnostics, Sokolská 581, 500 05 Hradec Králové, Czech Republic. Phone: (+ 420) 495 833 864; e‑mail: Helena.Kovarikova@fnhk.cz. ORCID ID: 0000-0001-5040-4140.


Original Article

Metabolic Tools for Identification of New Mutations of Enzymes Engaged in Purine Synthesis Leading to Neurological Impairment

(de novo purine synthesis / aminoimidazole ribotide / 5-formamidoimidazole-4-carboxamide ribotide /
PAICS / PFAS)

M. KRIJT, O. SOUCKOVA, V. BARESOVA, V. SKOPOVA, M. ZIKANOVA

Research Unit for Rare Diseases, Department of Paediatrics and Adolescent Medicine, First Faculty of
Medicine, Charles University and General University Hospital, Prague, Czech Republic

Pages: 152 - 157 (PDF)

Corresponding author: Marie Zikanova, Department of Paediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic,
Ke Karlovu 2, 121 08 Prague 2, Czech Republic, E-mail: marie.zikanova@lf1.cuni.cz