Volume 65 (2019) No. 3
Original Article
Reprogramming of Human Pancreatic Organoid Cells into Insulin-Producing β-Like Cells by Small Molecules and in Vitro Transcribed Modified mRNA Encoding Neurogenin 3 Transcription Factor
(diabetes / organoid / reprogramming / synthetic mRNA / in vitro transcription / IVT / neurogenin 3 / β-cell /
insulin / TGF / RepSox / small molecules)
T. KOBLAS1,3, I. LEONTOVYC1,3, S. LOUKOTOVA3, F. SAUDEK2
1Department of Experimental Medicine, 2Department of Diabetes, Institute for Clinical and Experimental
Medicine, Prague, Czech Republic
3First Faculty of Medicine, Charles University, Prague, Czech Republic
Pages: 109 - 123 (PDF)
Corresponding author: Tomas Koblas, Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, Prague, Czech Republic. Phone: (+420) 261 365 447; Fax: (+420) 261 362 820; e-mail: tomas.koblas@ikem.cz
Original Article
Enzymatic Isolation, Amplification and Characterization of Dental Pulp Stem Cells
(enzymatic digestion / dental pulp stem cells / trypsin / isolation)
N. PILBAUEROVA1, T. SOUKUP2, T. SUCHANKOVA KLEPLOVA1, J. SUCHANEK1
1Department of Dentistry, 2Department of Histology and Embryology, Charles University – Faculty of
Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic
Pages: 124 - 133 (PDF)
Corresponding author: Nela Pilbauerova, Department of Dentistry, University Hospital Hradec Králové, Sokolská 581, 500 05 Hradec Králové. Phone: (+420) 777245459; e-mail: nela.pilbauerova@lfhk.cuni.cz.
Original Article
Review of SRD5A3 Disease-Causing Sequence Variants and Ocular Findings in Steroid 5α-Reductase Type 3 Congenital Disorder of Glycosylation, and a Detailed New Case
(retinal dystrophy / SRD5A3-CDG / SRD5A3 / novel variant)
B. KOUSAL1,2, T. HONZÍK2, H. HANSÍKOVÁ2, N. ONDRUŠKOVÁ2, A. ČECHOVÁ2, M. TESAŘOVÁ2, V. STRÁNECKÝ2, M. MELIŠKA1, M. MICHAELIDES3,4, P. LIŠKOVÁ1,2
1Department of Ophthalmology, 2Department of Paediatrics and Adolescent Medicine, First Faculty of
Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic
3Moorfields Eye Hospital NHS Foundation Trust, London, United Kingdom
4UCL Institute of Ophthalmology, University College London, London, United Kingdom
Pages: 134 - 141 (PDF)
Corresponding author: Petra Liskova, Department of Paediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 2, 128 08 Prague 2, Czech Republic. Phone: (+420) 224 967 139; e-mail: petra.liskova@lf1.cuni.cz
Original Article
Deregulation of Selected MicroRNAs in Sinonasal Squamous Cell Carcinoma: Searching for Potential Prognostic Biomarkers
(biomarker / epigenetics / head and neck cancer / microRNA / sinonasal squamous cell carcinoma)
H. KOVAŘÍKOVÁ1, I. BARANOVÁ1, J. LACO2, K. ROZKOŠOVÁ2, H. VOŠMIKOVÁ2, M. VOŠMIK3, P. DUNDR4, K. NĚMEJCOVÁ4, J. MICHÁLEK5, V. PALIČKA1, M. CHMELAŘOVÁ1
1Institute of Clinical Biochemistry and Diagnostics, 2The Fingerland Department of Pathology, 3Department
of Oncology and Radiotherapy, Charles University, Faculty of Medicine in Hradec Králové and University
Hospital Hradec Králové, Czech Republic
4Department of Pathology, Charles University, First Faculty of Medicine and General University Hospital in
Prague, Czech Republic
5Department of Clinical and Molecular Pathology, Palacky University Olomouc, Faculty of Medicine and
Dentistry and University Hospital Olomouc, Czech Republic
Pages: 142 - 151 (PDF)
Corresponding author: Helena Kovaříková, Institute of Clinical Biochemistry and Diagnostics, Sokolská 581, 500 05 Hradec Králové, Czech Republic. Phone: (+ 420) 495 833 864; e‑mail: Helena.Kovarikova@fnhk.cz. ORCID ID: 0000-0001-5040-4140.
Original Article
Metabolic Tools for Identification of New Mutations of Enzymes Engaged in Purine Synthesis Leading to Neurological Impairment
(de novo purine synthesis / aminoimidazole ribotide / 5-formamidoimidazole-4-carboxamide ribotide /
PAICS / PFAS)
M. KRIJT, O. SOUCKOVA, V. BARESOVA, V. SKOPOVA, M. ZIKANOVA
Research Unit for Rare Diseases, Department of Paediatrics and Adolescent Medicine, First Faculty of
Medicine, Charles University and General University Hospital, Prague, Czech Republic
Pages: 152 - 157 (PDF)
Corresponding author: Marie Zikanova, Department of Paediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic,
Ke Karlovu 2, 121 08 Prague 2, Czech Republic, E-mail: marie.zikanova@lf1.cuni.cz
